Development of an optimized RNA-based murine norovirus reverse genetics system
نویسندگان
چکیده
منابع مشابه
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Human noroviruses are responsible for most cases of human gastroenteritis (GE) worldwide and are recurrent problem in environments where close person-to-person contact cannot be avoided (1, 2). During the last few years an increase in the incidence of outbreaks in hospitals has been reported, causing significant disruptions to their operational capacity as well as large economic losses. The ide...
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Human norovirus (HuNoV) is the leading cause of gastroenteritis worldwide. HuNoV replication studies have been hampered by the inability to grow the virus in cultured cells. The HuNoV genome is a positive-sense single-stranded RNA (ssRNA) molecule with three open reading frames (ORFs). We established a reverse genetics system driven by a mammalian promoter that functions without helper virus. T...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2010
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2010.07.006